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Reconstitution · Dosing Math · Storage

The Peptide Reconstitution Guide

Find your compound, get the exact amount of water to add and the exact number of units to draw, then mix it and store it right. Built in the order you actually work in, not the order a textbook would list. One page, one email, no login.

For educational and research purposes only. This is not medical advice and is not human-use guidance. Dose figures are research reference ranges, not instructions. Consult a qualified professional before acting on anything here.

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The full reconstitution guide. Every compound, every number.

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Calculate your peptide and plan your cycle

The full tool covers more compounds than the tables below and does the whole job in one place: concentration, units to draw, doses per vial, and full cycle planning. Skip the guide and go straight to your numbers.

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Find Your Compound

Search your compound and read the row. It gives you the reference water amount, the reference dose and how often, the units to draw on a U-100 insulin syringe, how many doses a vial holds, and the route. If you already know what you are holding, this row is the whole answer. If your vial size or water amount is different, use the calculator in the next section.

Reference planning sheet, SubQ compounds (2mL BAC)

PeptideEff. dose ref.FrequencyBAC volVol / doseDays / vialMin cycleMax cycle
AOD-9604 (10mg)350 mcg1x/day2.0mL0.07mL / 7u2828d56d
BPC-157 (5mg)250 mcg1x/day1.5mL0.075mL / 7.5u2014d56d
CJC-1295 No DAC (5mg)100–200 mcg2x/day2.0mL0.04mL / 4u2528d56d
CJC-1295 No DAC (10mg)100–200 mcg2x/day2.0mL0.02mL / 2u50100d100d
DSIP (5mg)300 mcg1x/night1.5mL0.09mL / 9u16.714d42d
Epithalon (10mg)5–10 mg1x/day2.0mL1.0mL / 100u2–410d20d
GHK-Cu (50mg)2 mg1x/day5.0mL0.20mL / 20u2528d56d
GHRP-6 (10mg)100 mcg2x/day2.0mL0.02mL / 2u5028d56d
Gonadorelin (2mg)100 mcg2x/week2.0mL0.10mL / 10u208w16w
HCG (5,000 IU)500 IU2x/week5.0mL0.50mL / 50u3528d56d
Ipamorelin (5mg)200 mcg2x/day2.0mL0.08mL / 8u12.528d56d
Ipamorelin (10mg)200 mcg2x/day2.0mL0.04mL / 4u2528d56d
KPV (10mg)250–500 mcg1x/day2.0mL0.05mL / 5u20–4028d56d
LL-37 (5mg)100–200 mcg1x/day1.5mL0.03mL / 3u25–5014d28d
MOTS-c (10mg)500 mcg1x/day2.0mL0.10mL / 10u2028d56d
NAD+ (500mg)50 mg2x/week5.0mL0.50mL / 50u3528d56d
Oxytocin (2mg)0.15 mg1x/day2.0mL0.15mL / 15u13.328d56d
PT-141 (10mg)1.25 mg2x/week2.0mL0.25mL / 25u2814d56d
Retatrutide (5mg)1 mg2x/week1.0mL0.20mL / 20u17.528d84d
Retatrutide (10mg)1 mg2x/week2.0mL0.20mL / 20u3528d84d
Retatrutide (15mg)1 mg2x/week3.0mL0.20mL / 20u52.528d84d
Retatrutide (20mg)1 mg2x/week3.0mL0.15mL / 15u7028d84d
Semax (10mg)300 mcg2x/day1.0mL0.03mL / 3u1614d28d
Selank (10mg)300 mcg2x/day1.0mL0.03mL / 3u1614d28d
SS-31 (5mg)1–2 mg1x/day2.0mL0.40mL / 40u5–1014d28d
TB-500 (5mg)2 mg1x/week2.0mL0.80mL / 80u6.2528d56d
Tesamorelin (5mg)1 mg1x/day2.5mL0.50mL / 50u528d56d
Tesamorelin (10mg)1 mg1x/day2.5mL0.25mL / 25u1028d56d
Thymosin Alpha-1 (5mg)900 mcg2x/week2.0mL0.36mL / 36u13.928d84d
Wolverine Blend 10mg250 mcg ea.1x/day2.0mL0.10mL / 10u2028d56d
Wolverine Blend 20mg250 mcg ea.1x/day2.0mL0.05mL / 5u4028d56d

Values are general research reference ranges only, not dosing instructions for human use. Ranges show the low end in the Vol per dose column. Epithalon is dosed in milligrams, so its draw is a large volume that empties a vial in a few days. Confirm your intended dose before relying on any figure.

How to read your syringe. On a standard U-100 insulin syringe, 100 units equals 1mL. Units and milliliters are not two systems. Units are simply the marks on that 1mL scale. Once you know the concentration, the unit line is just the liquid volume you are pulling. The syringe does not know the compound dose, it only knows the liquid amount.

10u0.1 mL
20u0.2 mL
25u0.25 mL
50u0.5 mL

Compound reference by route

Same compounds, grouped by how each one is most commonly administered in research contexts. Some appear in more than one group because research has studied them through several routes with different effects at each site. Every range here is general research reference data, not instructions for human use.

Subcutaneous (SubQ), injected under the skin

Usually drawn from a reconstituted vial with a U-100 insulin syringe and injected into subcutaneous (under-skin) tissue, commonly the abdomen or thigh.

CompoundResearch dose rangeFrequencyPrimary mechanism (plain English)
AOD-9604250–500 mcgDaily / fastedA fragment of the GH sequence studied for fat-mobilization logic rather than GH replacement.
BPC-157250–500 mcg1–2x/dayStudied for gut-lining support and local tissue repair signaling, especially where inflammation or soft-tissue stress is part of the picture.
CJC-1295 (No DAC)100–300 mcg1–2x/day, fastedA GHRH analog studied to support the body's own GH pulse rather than supplying outside growth hormone.
DSIP100–300 mcgNightlyResearched for sleep architecture support, especially when deep-sleep quality looks like the limiting variable.
Epithalon5–10 mgNightly / splitStudied for pineal and melatonin-related signaling, usually in longevity research rather than acute physique use.
GHK-Cu1–2 mgDaily / several x/wkA copper peptide studied for tissue quality, skin remodeling, and repair signaling rather than fat loss directly.
GHRP-6100–300 mcg1–3x/day, fastedA ghrelin receptor agonist studied to amplify GH release, but also known for a strong hunger signal.
Gonadorelin100–300 mcg2–3x/weekStudied for hypothalamic-pituitary-gonadal (hormone axis) signaling where suppression, flat mood, and low drive are part of the case.
HCG250–500 IU2–3x/weekStudied for testicular signaling support in hormone axis research. Not a peptide, but commonly grouped with peptide protocols.
Ipamorelin100–300 mcg1–2x/day, fastedA ghrelin mimetic studied to amplify GH pulse signaling with less appetite drive than older GHRPs.
Kisspeptin-10100–300 mcg2–3x/weekStudied for upstream reproductive hormone signaling and hypothalamic control rather than direct body composition.
KPV250–500 mcg1–2x/dayA short anti-inflammatory peptide studied where gut irritation and systemic inflammatory signaling overlap. Also oral.
LL-37100–250 mcgDaily / several x/wkAn antimicrobial peptide studied in immune and wound-related research, usually handled with more caution than standard repair peptides.
MOTS-c5–15 mg2–3x/weekA mitochondrial (cell energy) signaling peptide studied for energy handling and AMPK-related adaptation rather than appetite suppression.
NAD+25–100 mg2–5x/weekA redox cofactor (cell fuel helper) studied for output capacity when fatigue is part of the bottleneck.
Oxytocin Acetate100–300 mcgAs scheduledStudied for social bonding, stress regulation, and sometimes appetite-related signaling. Context matters heavily here.
PT-141 (Bremelanotide)0.5–2 mgAs neededA melanocortin agonist studied mainly for arousal signaling, not as a body composition tool. Also intranasal.
Retatrutide1–8 mgWeeklyA triple agonist studied for intake control plus output pressure through glucagon signaling, which changes how hunger and heat are read.
Semax300–900 mcgDaily / splitA nootropic (cognition) peptide researched for cognitive signaling. Some setups study SubQ delivery alongside the more common intranasal route.
Selank250–500 mcgDaily / splitAn anxiolytic (anti-anxiety) peptide more often studied intranasally, with SubQ use appearing in some research contexts.
SS-31 (Elamipretide)5–20 mgDailyA mitochondrial peptide studied to stabilize inner-membrane function when energy inefficiency, not appetite, is the actual problem.
TB-5002–5 mgWeekly / splitA systemic repair peptide studied for cell migration and recovery signaling across soft tissue and connective tissue.
Tesamorelin1–2 mgDailyA GHRH analog studied for stronger, more measurable GH pathway pressure, often when visceral fat and lean-mass protection are central.
Thymosin Alpha-10.5–1.5 mg2–3x/weekAn immune-signaling peptide studied when recovery, inflammation, and immune resilience overlap.
Wolverine Blend (BPC+TB-500)Varies by blendDaily + weeklyA combination used in repair research to pair local gut and tissue signaling with broader systemic recovery.
Intranasal, administered via nasal spray

Used most in nootropic, anxiolytic, and certain specialty signaling contexts where fast, non-injectable delivery is part of the research setup.

CompoundResearch dose rangeFrequencyPrimary mechanism
Semax300–900 mcgDaily / splitStudied for BDNF-related cognitive signaling, especially where mental flatness and reduced focus sit inside a larger recovery problem.
Selank250–500 mcgDaily / splitStudied for stress and anxiety regulation without the sedation profile many researchers try to avoid.
PT-141 (Bremelanotide)1–5 mg equiv.IntermittentAn alternate route for melanocortin signaling research, same basic mechanism but a different onset profile.
Oxytocin100–300 mcgIntermittent / dailyStudied for bonding, calmness, and stress-related signaling. Interpretation depends heavily on context and intent.
CerebrolysinProtocol dependentProtocol dependentOften discussed in neural recovery circles. Intranasal discussion exists but the evidence is uneven. Not a peptide in the narrow sense.
Dihexa5–20 mgDailyA research-stage cognitive compound discussed for synaptic and learning-related signaling. Still highly experimental with limited human data.
PE-22-28400–1000 mcgDailyAn experimental anxiolytic peptide discussed in nootropic communities with limited formal human data.
NA-1 (Nerinetide)Protocol dependentAcute / short blockStroke and neuroprotection literature drives most discussion here. Not a casual peptide reference item.
Oral, taken by mouth

Included because many researchers group these with peptide workflows even when the compound itself is not technically a peptide.

CompoundResearch dose rangeFrequencyNotes
BPC-157 (oral form)250–1000 mcg1–2x/dayThe oral form is usually studied for GI-targeted support rather than the broader repair logic often assumed from injectable use.
KPV250–1000 mcg1–2x/dayOral use is usually discussed when gut inflammation is the leading issue rather than systemic performance. Also SubQ.
Larazotide (AT-1001)0.25–1 mg1–3x/dayA tight-junction modulator (gut barrier) studied in gut research rather than physique applications.
MK-677 (Ibutamoren)10–25 mgDailyNot a peptide. An oral secretagogue studied for sustained GH and IGF-1 pathway stimulation through ghrelin receptor signaling.
SLU-PP-33250–200 mcg (early discussion)Daily, research stageResearch suggests ERR pathway activation may influence mitochondrial biogenesis and metabolic output. Human data remains extremely limited as of early 2026.

Your mix

Your Exact Numbers

Pick your compound to autofill the reference vial size, water, and dose, then adjust anything that does not match what you are holding. Or type all three yourself. It returns the concentration and the units to draw on a U-100 syringe. It converts the numbers you enter. It does not tell you what dose to take.

Concentration 3.333 mg/mL 33.33 mcg per unit
Draw this 7.5 units 0.075 mL
Doses per vial 20 at this dose

Presets fill the reference vial size, water, and the low end of the dose range. They are editable starting points, not recommendations. U-100 means 100 units equals 1 mL. Change the water and every unit line changes with it. All figures are research reference math only.

Reconstitution reference (2mL BAC / 200 units)

Vial sizeBAC waterDoseDraw (U-100)Doses / vial
2 mg2.0 mL100 mcg10 units20
2 mg2.0 mL200 mcg20 units10
5 mg2.0 mL250 mcg10 units20
5 mg2.0 mL500 mcg20 units10
10 mg2.0 mL500 mcg10 units20
10 mg2.0 mL1 mg (1000 mcg)20 units10
15 mg2.0 mL1.5 mg20 units10
20 mg2.0 mL1 mg (1000 mcg)10 units20
20 mg2.0 mL2 mg20 units10

Before you mix

The Critical Gear List

The point of the gear list is not to sound technical. It is to remove avoidable errors. Most dosing confusion starts before a vial is ever mixed, because the wrong tool changes the handling, the pressure, and the accuracy of the whole setup.

The 29-gauge (29G) needle

A 29G insulin-style needle is a common default because it balances control against stopper wear. It is narrow enough to limit damage to the rubber vial top while still being practical for handling pressure. Thinner needles such as 31G can struggle to equalize pressure in some vial setups, which is a handling quirk, not a hard rule.

The 3mL reconstitution syringe

A 3mL syringe makes the water transfer easier to read and easier to control. It also stops you reaching for the same tiny dosing syringe to do both the mixing and the micro-dose math. The tool used to mix should not be the tool used to read small unit pulls.

The single-entry principle

Where you can, draw the full water amount once and finish the mix in one controlled entry. Fewer punctures means less stopper wear, less handling error, and a cleaner process. Repeated tiny entries just create more chances for pressure problems and contamination.

Bacteriostatic (BAC) water

For a vial you will use more than once, bacteriostatic water is generally preferred because the benzyl alcohol (a mild preservative) helps limit bacterial growth over time. Sterile water is not the same thing. It can be fine for a single-use research context, but for repeated entry, BAC water is the cleaner practical choice.

The method

The Slow Drip Rule

Lyophilized (freeze-dried) powder looks stable because it is dry. The moment water goes in, handling starts to matter. The standard handling concern is that blasting water straight onto the powder puck may disturb the peptide and cost a little potency. This is closer to careful-handling convention than settled data for most short peptides, and one fast push does not automatically ruin a vial. The cleaner method is simply the more rational one, so it is the one worth building as a habit.

  1. 01
    Equalize pressure

    Pull a little air in first so the vial does not fight the transfer. Pressure problems are what make people push too hard.

  2. 02
    Tilt the vial

    Angling it gives the water a glass wall to run down instead of dropping straight onto the powder.

  3. 03
    Aim at the glass wall

    Do not blast the puck. Let the liquid run down the side and settle gradually.

  4. 04
    Push slowly

    The right speed feels almost boring. A slow transfer lets the solution spread without turning into turbulence.

  5. 05
    Swirl gently, do not shake

    Skip hard shaking. A gentle swirl or slow roll dissolves the powder while keeping the structure intact.

Common reconstitution mistakes

Wrong BAC water volume

The biggest math error in the category. If the water amount changes, every unit line changes. A correct vial amount with the wrong water still gives you the wrong syringe read.

Sterile water instead of BAC water

For a single use they can sound interchangeable, but for a multi-use vial the contamination logic differs. The issue is not the word sterile. It is repeated entry over time.

Shaking instead of rolling or swirling

Hard shaking is usually a sign the process got rushed. Gentle movement is the cleaner standard.

Storing reconstituted vials at room temperature

Most reconstituted peptides are treated as refrigerated materials. Leaving them out adds avoidable degradation and shortens useful life.

Using the dosing syringe to reconstitute

The syringe that reads 5, 10, or 20 unit pulls should not be the one transferring the full water volume. That is how small reading errors get baked in from the start.

After you mix

Storage and Safety

Temperature and light

Keep reconstituted vials refrigerated, out of light, and handled as if every extra touch lowers quality. Clean stopper technique, fewer entries, and calm handling matter more than people think, because small mechanical errors add up over time.

Shelf life, unconstituted

Dry peptides are commonly stored frozen for months, sometimes longer, depending on the compound and handling quality. Once out of the freezer, keep temperature swings to a minimum before you mix.

Shelf life, reconstituted

Once wet, most research setups treat 14 to 28 days refrigerated as the conservative window for most peptides, with certain compounds discussed as shorter or more fragile exceptions. Always confirm per compound.

The broader lesson

Concentration and preparation errors create fake protocol problems. Someone can misread a compound when the real issue was the mix, the syringe math, the storage, or the handling. That is why mechanics come first.

Concentration and preparation errors create fake protocol problems. A researcher can blame a compound when the real problem was the mix, the syringe math, the storage, or the handling. Get the mechanics right first, then judge the compound.

Next step

When the setup is right but the results are not

This guide fixes preparation. It does not diagnose why a protocol stalled, why hunger changed, why energy dropped, or why a stack got harder to read. The Protocol Bottleneck Tool does. Answer a short set of questions about your current protocol and it maps your situation across seven bottleneck categories, then tells you where the friction most likely is and what to look at first. It takes about three minutes, it is free, and it does not ask what to dose or point you at a vendor.

For educational and research purposes only. Not medical advice. Not human-use guidance. Dose figures are research reference ranges, not instructions. project-theo.com